A Molecular Imprinted Polymer Sensor for Biomonitoring of Fenamiphos Pesticide Metabolite Fenamiphos Sulfoxide

A new electrochemical method for the identification and quantification of Fenamiphos pesticide's major metabolite in biological samples – Sulphoxide (FNX) was developed. Computational calculations, Density Functional Theory (DFT) semi-empirical models (PM3) were performed to determine best monomer, pyrrole, a ratio 1 : 5 (template: monomer) chosen fabrication FNX?MIP sensor obtained by electropolymerization. The responded well increasing FNX concentrations (range 1–30 ?M). Limit detection (LOD=0.183 ?M, LOQ=0.601 ?M), respectively, selectivity, repeatability also investigated developed method. percentage recovery showed good agreement compared reference values from GC-MS, which used as FXN?MIP proved selective presence potential interferents. successfully applied determination spiked plasma urine matrixes with acceptable rates. proposed successful detecting prepared vitro metabolism FNP using liver microsomes metabolize it.